Inhibition of protease activity and anti‐quorum sensing of the potential fraction of ethanolic extract from Sansevieria trifasciata Prain leaves against Pseudomonas aeruginosa

Sansevieria trifasciata is a plant that commonly utilized in traditional medicine. The leaves of S. show antibacterial properties against Pseudomonas aeruginosa. This bacterium an opportunistic pathogen can cause serious illness humans and produce variety virulence factors responsible for bacterial pathogenesis with quorum sensing (QS) systems mediate intracellular communication. Bacteria protease through QS mechanism which they express signaling molecules to become pathogens. Proteases are extracellular enzymes required successful infection biofilm spread regulate cellular physiological functions. research aimed evaluate the protease, anti‐QS activities ethanolic extract from P. aeruginosa expression genes. An azocasein test was used determine activity qualitative quantitative methods. Using real‐time polymerase chain reaction, study conducted investigate effect on selected QS‐regulatory genes at transcriptional level. results showed potential inhibited enzyme by as much 77.1%. decreased expressions lasA, lasB, lasI, lasR, rhlI, rhlR 2‐ΔΔCt values 0.81, 0.93, 0.76, 0.97, 0.90, 0.55 respectively.